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In addition, the Harvard and Cincinnati groups both reported a high false-positive rate (∼70%) among identified variants, thus requiring confirmatory sequencing which is time-consuming and adds cost [27].
Novel sequence variants identified only in controls in this study were confirmed either by sequencing in the opposite direction or by a second confirmatory sequencing reaction in the same direction as the first.
HC carried out confirmatory sequencing experiments.
The accuracy of the cloned DNA inserts was verified by confirmatory sequencing (Macrogen).
The original genomic DNA was used for confirmatory sequencing of detected SNPs and for genotyping the common SNPs using the TaqMan® assay.
MK performed further MethyLight assays as well as normal and digital MS-HRM assays, confirmatory sequencing, and assisted in writing the paper.
Similar(42)
It is currently recognised that the utility of DCE in clinical practice is mainly as a confirmatory sequence for PZ lesions [58]; however, further studies are required to determine the value of DCE in PCa.
With this strategy, confirmatory sequence in frozen samples could be performed over 97% of the time.
LCMV was diagnosed based on seroconversion and positive PCR results with confirmatory sequence data (6 ).
For available samples that tested positive by PCR for the EML pathogen during 2009 2013, confirmatory sequence analysis was performed by using a 3730 DNA Analyzer (Applied Biosystems, Foster City, CA, USA) and analyzed by using Sequencher DNA sequence analysis software version 4.2 (Gene Codes Corporation, Ann Arbor, MI, USA).
Sequencing of three NA OPXV genomes was carried out using standard protocols of the Roche GS-20 pyrosequencing platform (Roche Applied Science, Indianpolis, IN) and confirmatory Sanger sequencing.
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