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Three large-volume 'control' specimens (obtained from blood donations, and chosen to represent a range of serological responses) were supplied to laboratory technicians with each panel, for regular confirmation of reproducibility and stability of assays.
We performed our ChIP-seq experiment with a higher number of individual biological replicates than are typically used in genome-wide location analysis, and developed a novel peak-filtering strategy based on both computational analysis and independent experimental confirmation of reproducibility.
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The speed of confirmation and reproducibility of the findings has certainly been unprecedented.
Each PCR was performed at least two times, for confirmation of PCR specificity and reproducibility.
The importance of the study is the confirmation that diagnostic reproducibility of minimally invasive FTC is low and that this has clinical implications, and also implications for the design of studies into the treatment and outcome of FTC.
Once a good reproducibility was obtained, the confirmation of the differences in COI expression was assessed using the 9 biological replicates per ecotype.
Once a good reproducibility was obtained, the confirmation of the differential COI expression was assessed using the 9 biological replicates, and tubulin and histone as reference genes for the normalization.
Experiments usually escape the test of reproducibility unless they prove something particularly important, controversial or commercialisable.
As it has been clarified before, reproducibility of results is just one of many aspects of reproducibility.
A high degree of reproducibility of phase analysis is observed.
"The Economics of Reproducibility in Preclinical Research" PLoS Biology 13 6).
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