Sentence examples for Confirmation by sequencing from inspiring English sources

Candidate constructs were identified by colony PCR, restriction digestion mapping and further confirmation by sequencing.

We thank M. Fink and W. Giles for coding the automated extraction of heart beat data from traces, J. Ayroles and E. Kennerly for the SFP analysis, and P. Hunt for linkage confirmation by sequencing.

Specifically, a C was removed from the sequence, CAGACGGCGTACACCACCGGCTCCGCCCGAcCGCCGGATGTGCCGCAACTCCAACCTGGCC, without confirmation by sequencing.

The deviation motivated a careful revision of the genotypes, and their confirmation by sequencing.

For fragments that do not contain a microsatellite, the possible polymorphism was firstly determined by high-resolution melting analysis on a LightCycler 480 (Roche), before confirmation by sequencing.

After confirmation by sequencing, the fused vector and empty vector were transiently transformed.

Instead, in the past a number of prescreening methods have been developed and applied in order to scan amplicons in large (mutant) populations for the presence of sequence polymorphisms, prior to confirmation by Sanger sequencing.

Since the presence of 2C and HA epitopes was not confirmed serologically (Fig. 6d2), their presence relied on the confirmation by sequence analysis of the constructs and the observation that the size of the chimeric proteins detected using anti-CCMV serum corresponded to the expected size of the predicted protein.

Only the ENO2 gene, which was hypermethylated in four out of five pairs, was found to have good PCR amplification, which was consistent with the outcome of the MeDIP (Fig.  3) and that could be subjected to further confirmation by BSP sequencing.

The resulting plasmids pMAL-c2-exochitinase and pMAL-c2-endochitinase were transformed into JM109 E. coli, followed by sequencing confirmation of in-frame gene insertion into the pMAl-cX2 plasmid.

Nine standard strains of different serovars commonly found in China and the avirulent strain of L. interrogans serovar Lai (Table 2) were selected to analyze their l-paf-ah gene including their probable promoter regions via PCR amplification followed by sequencing confirmation.