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To confirm the quantity of viral copies in the diluted HIV-1 infected ACH-2 cell suspension, at a later date, the DNA was extracted using the Qiagen DNA extraction kit, and HIV copy number was quantified using HIV-1 pol real-time PCR [ 14, 15].
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As control, reactions for eIF2α were run to confirm the uniform quantity and quality of samples.
In the classic assessment, children are presented with two identical beakers of liquid that are filled to the same level with water, and asked to confirm that the quantity of water is equivalent across the two beakers.
A vario Micro cube CHNS analyzer was used for a subset of OC-bearing ferrihydrite samples to confirm that the quantity of sorbed C in the solid phase was consistent with that removed from the liquid phase before and after reaction (data not shown).
In the cases where the estimates did not have face validity, controls were made to ensure that there had not been mistakes with coding, and discussions were held with the regional costing experts to confirm the basis of their quantity and price estimates.
Despite the high spatial and temporal variability of burned area, this study confirms the large quantities of biomass from fire-killed stands available as ecologically sustainable feedstock for bioenergy, which, combined with the comparatively smaller and more stable quantities from clearcut harvesting residues could supply theoretical pellet plants.
The blots are presented to confirm the presence of the receptors, but not the quantity or different expression levels.
Fragments smaller than 300 bp were removed and the purified cDNA samples were assesed on DNA chip (Agilent 2100 Bioanalyzer, USA) to analyze quantity as well as confirm the fragment size (350 800 bp).
The interviews confirm the above observations, and stress that in relation to fuel quantity, especially fuel availability is important.
In this paper, firstly, method of confirming the maximal effective quantity of reducing duration is designed; secondly, on the basis of above method and the method of mending the minimal cost, the main method of reducing duration directly is designed to solve time-cost tradeoff problem, and by analyzing validity of the method, the method could obtain more optimal result for the problem.
Measurements for the 50 μl extracts confirmed the low DNA quantity at 1.86 ng/μl and <0.10 ng/ul respectively, heavy fragmentation to 51 bp median length was observed for the first feather.
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