Immunohistochemical staining was visualised using streptavidin biotin peroxidase complex kit reagents (BioGenex, San Ramon, CA, USA) with diaminobenzidine/H2O2 as chromogenic substrate.
The looped, hocketing rhythms that drummer Duncan Bellamy coaxed from his complex kit were reminiscent of early (lo-fi) Kraftwerk and the organic techno of Brandt Brauer Frick.
The complex kit comprises 87 pieces including three bridges, two tunnels, cargo cranes, stations and little plastic people.
Now consider Bloodhound SSC, which is the biggest and most complex kit you can imagine.
Antibody binding was detected using Vectastain Elite avidin-biotin complex kit (VectorLabs) and 0.1% diaminobenzene (Sigma, St . Louis MO).
Following incubation, the sections were washed 3 times in 1× TBS containing 0.1% Triton X-100 and incubated with biotinylated secondary antibody, as detailed in the Vectastain Avidin Biotin complex kit (Vector Laboratories, Burlingame, CA), and were visualized by 3'3'-diaminobenzidine (DAB, Sigma).
Staining was visualized using the Vectastain avidin biotin complex kit and diaminobenzidine peroxidase substrate kit (Vector Laboratories).
19 In brief, Vectastain Elite avidin-biotin complex kit (Vector Laboratories) was used for immunohistochemistry.
After repeated washings with PBS, sections were incubated with avidin-biotin complex kit (ABC-peroxidase, Dako).
The sections were incubated in avidin:biotinylated enzyme complex kit and exposed to 3,3′-diaminobenzidine kit for detection.
