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Sequencing of the complete intervening regions confirmed the suspected synteny within the same mating type across the majority of the species (Additional File 2).
Intronic primers were designed to amplify exons 3 29 of FU either as single exons with flanking intronic sequences or as products containing two exons with flanking intronic sequence and the complete intervening intron.
Furthermore, the complete intervening sequence between nad4 and rrnL in the large SA1 chromosome was validated using capillary sequencing primers (primer pairs 5 8 on page 2 of Additional file 1) and it was confirmed that no duplicate nad6 gene was present.
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The compiled sequence extended from the 3′-terminal region of exon 81 to the 5′ end of exon 94, providing novel data completing intervening sequencing gaps and confirming transcription of the included intronic segments.
The use of standardised patients also demonstrated the possibility of harm to patients if the study was observing 'real' patients which would have rendered it difficult to complete without intervening.
For example, Pan_1_A, Pan_2_B and Pan_3_C, which are the chimpanzee orthologues of the three exons of Homo_1, appear consecutively within the chimpanzee genome sequence, complete with intervening intronic sequence, and thus were assigned as a full length chimpanzee PRAME, Pan_1.
Longicornis males readily completed the intervening steps and displayed for both giraulti (96% of total males) and longicornis (100%) females, and giraulti males readily completing the intervening steps and displayed for longicornis (90%) and giraulti (100%) females.
The remaining archaeal primers evaluated here did not directly overlap with currently known introns; however, depending on the chosen complementary primer for PCR, the resulting amplicons may contain complete or partial intervening intron sequences.
There was a wide range of comfort and experiences among the participants for asking smokers to adhere to the SF zones, ranging from complete confidence in intervening with a smoker (more common among key informants) to feeling a risk of offending the smoker (more common among community members).
In their paper, the researchers compared their results with those of the last comprehensive ape survey, which was done between 1981 and 1983, as well as with results from smaller surveys completed in the intervening years.
Several large, randomized controlled trials have been completed in the intervening decade.
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