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Cold challenge did not alter Txnip expression in the murine hypothalamus or liver but significantly increased expression in WAT and significantly decreased expression in BAT of both genotypes (P <.05, WAT; P <.01, BAT).

(A D) The wildtype mice were subject to cold challenge.

(A E) The wildtype mice were subject to cold challenge.

Open image in new window Figure 1 Intra-adipose sympathetic plasticity in response to cold challenge.

(D F) Adrb1−/−; Adrb2−/−; Adrb3−/− mice or control mice (Adrb1+/−; Adrb2+/−; Adrb3+/+) were subject to cold challenge.

(C H) TrkAF592A/F592A mice daily-treated with 1-NaPP1 or vehicle control were subject to cold challenge.

(A and B) The wildtype mice were subject to cold challenge.

We next examined the mechanism regulating NGF expression in response to cold challenge.

The wildtype mice were subject to cold challenge, and then recovered at thermal-neutral condition (32 °C).

Furthermore, we show that NGF expression in WAT depends on the catecholamine signal in cold challenge.

We further show that cold-elicited NGF expression in WAT depends on the catecholamine signal during cold challenge.

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