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A second scan was performed with the 568 nm line, and the co-localization of cells was recorded.
Additionally, co-localization of cells expressing RasGRF1 and MMP-1 is most apparent in the synovial sublining layer.
To analyze sections for co-localization of cells, photographs were taken using a digital camera that was connected to a laser-scanning confocal microscope (Carl Zeiss, Oberkochen, Germany).
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Instead reorganization, clustering and co-localization of cell surface molecules were found to initiate the signalling process [ 18, 19].
For clarity, the inset panels show a single z-slice image of partial islet showing no co-localization of cell type specific reporters.
To investigate the specific role of disrupted autophagy flux in neuronal cell death, we quantified co-localization of cell death markers with p62 in the gray matter of the spinal cord by IHC staining.
We observed a strong positive relationship between macrophages and/or CD8 and P24 positive cells by rank correlation analysis (p < 0.05), although the co-localization of CD68+ cells with P24+ cells was much more pronounced (Fig. 2b and 2d), whereas only to a lesser degree were CD8+ T cells and P24 antigen+ cells were seen to be co-localizing in these two severe HAD patients (Fig. 2a and 2c).
Since the majority of the T cells in the salivary glands of primary SS patients are CD4+ T cells, and the IL-21 produced by activated CD4+ T cells is known to up-regulate their expression of CXCR5 [ 16], IL-21 possibly promotes the co-localization of T cells and B cells in the functional ectopic germinal centers in the salivary glands of SS patients, which in turn elevates autoantibody production.
Panel H, an overlay of panels F and G, demonstrates co-localization of HB1.F3.C1 cells (orange/yellow, indicated by white arrows) and tumor cells (green), at sites immediately adjacent to and distant from the blood vessel (bv).
Hyperspectral darkfield images [ 12] and phase contrast images (Fig. 6 ) of the cells incubated with NPs, taken prior to trypsinization, show the co-localization of the cells and particles, indicating the cellular uptake of the particles.
(D) Confocal imaging for the KO treated group displays co-localization of TH+ cells with DAT+ neurons (scale bar 50 μm).
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com