Sentence examples similar to Clone utilizing from inspiring English sources

The purified fragments were cloned utilizing the PGEM-T Easy Vector kit (Promega).

Species of origin and details of clones utilized are given in the legend to Figure 1.

Traditionally, map-based cloning utilizes DNA-based markers that have been mapped to fairly precise areas of plant genomes.

Notably, significant numbers of expanded adult CD5+ B cells, and fetus and SLE Vλ clones utilized H-joining at the junctions.

Sequencing of insert-positive clones utilized labeled M13 forward and reverse primers and a LI-COR sequencer at the Centre for Biomedical Research at the University of Victoria.

Recombinase-dependent cloning utilizes recombinase as the cloning enzyme to catalyze the fusion of the insert into the target vector with homologous ends, but it is generally provided as proprietary components of commercial kits, which can be costly [ 4, 5].

Consistently, the two clones utilize an arginine to make base specific contacts with the guanines in the mCpG site, reminiscent of what has been observed for both the MBD and zinc finger protein structures.

The current study will provide a basis to clone and utilizing the gene that is responsible for the albino trait in the future.

The software has been designed to automatically generate and maintain a standardized nomenclature during all steps of the cloning process, utilizing unique and consistent identifiers for ORFs, PCR reactions, colony-PCRs, entry- and expression clones (Fig. 7).

Mutations were introduced by overlap PCR, and the amplicon were inserted to the cDNA clones through utilizing the inner restriction enzyme sites of the vectors.

Many efforts have been made to construct such as strain by cloning pentose utilizing genes into ethanologenic microbes (Hahn- Hägerdal et al. 2007).