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Phi29-DNA polymerase was supplied by Epicentre Biotechnologies (10,000 U/mL).
Buccal samples were collected using a MasterAmp Buccal Swab Brush by Epicentre Technologies of Madison, WI.
The poor yield obtained with the protocol proposed by Epicentre is due to amplification failures since the quality of the commercial RNA has been checked.
Four oligos described in the Nextera DNA sample prep kit (Epicentre and Illumina) were added to the every well containing purified fragmented DNA in concentrations recommended by Epicentre.
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After rRNA depletion, samples were purified using a Qiagen RNeasy MinElute Cleanup Kit according to the instructions outlined by the Epicentre Ribo-Zero™ Magnetic Kit.
Strikingly, it is possible to identify the tremblor's epicentre by measuring the disrupted sleep suffered by thousands of people in the area who use a bracelet pedometer and sleep-tracking device made by Jawbone (see chart below).
A ∼10.5 kb transgene cassette was excised by digestion with KpnI and DrdI and purified by GELase extraction (Epicentre Biotechnologies, Madison, WI, USA).
The recent quakes have reshaped more than 230 square miles of land, lowering areas around the epicentre by up to 70 cm, according to data released by Italy's National Institute for Geophysics and Volcanology (INGV).
The anomalous changes in the ionosphere are observed firstly over a distant station (wuhn, ∼0800 1600 UT) during afternoon to midnight hours and few hours later over the near-by stations to the epicentre in the nighttime (∼1500 2300 UT, ∼2100 0500 LT).
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