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Brief current pulses (50 μs, 1 40 μA) were delivered with a stimulation-isolation unit (Nihon Kohden, Tokyo, Japan).
(g) Phase plots of APs from the non-silence shRNA and Cdk5 shRNA rats 1 day after CFA evoked by brief current injections.
(i) Phase plots of the APs from the naive rats and the rats 1 day after CFA evoked by brief current injections.
A brief current pulse of sufficient amplitude applied to a single node of Ranvier should be able to elicit a spike in a myelinated axon model.
Large amplitude muscle contractions, recorded from ROI (yellow), were evoked by brief current pulses (upper trace, black arrow and dotted line, 3.2 mA, 120 µs-long) applied at 30, 60, 75, 90, 105, 120, 135 s.
But what difference does it make if a perturbing current is generated by a mini-epsp or ipsp, or the closing or opening of a channel, or injection of a brief current pulse through a microelectrode?
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Transcranial Magnetic Stimulation (TMS): TMS is a useful and safe tool that sends brief currents via an insulated coil to painlessly generate currents in the brain tissue beneath the coil.
We note that the choice of emulating stimuli with brief currents rather than detailed synaptic input served to (i) trigger a somatic spike robustly and (ii) keep the analysis at the fundamental level of response-current (f I), thereby allowing our findings to benefit from being general and not tied to specific assumptions regarding the synaptic input.
Thinking that the brief tail current recorded from WT CLC-5 might be a QOFF gating current, we integrated and analyzed these further.
Herein "Techniques in biotechnology: essential for industrial applications" gives an edge to understand brief history, current applications, and techniques of industrial biotechnology.
The largest amplitude is the initial response to a brief weak current pulse.
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