Sentence examples for Before polymerase from inspiring English sources

Before polymerase chain reaction based HPV genotyping, proteinase K was inactivated at 95°C for 15 minutes.

Before polymerase chain reaction (PCR) amplification of a 93-base-pair segment of exon 7 of TP53 encompassing codon 249, 228 copies of an internal standard plasmid were added to all DNA extracts to provide a reference for quantitation.

In particular, the β-ssDNA contact may function as a 'placeholder' that keeps β-clamp near the 3′ end of primed DNA before polymerase binding and initiation of DNA replication6.

Samples were stored at −20°C before polymerase chain reaction (PCR) amplification was performed.

The high fidelity of mtDNA replication (Table 1) is largely determined by the N-terminal domain, which exhibits 3′→5′ exonuclease activity for the excision of misincorporated bases before polymerase extension.

All work phases before polymerase chain reaction (PCR) were conducted in a laboratory where no DNA handling was previously conducted and where no physical connection to other laboratories existed.

When we start with small populations we see, as before, that polymerase rate rapidly evolves to its equilibrium value (fig. 4B).

Genomic DNA isolated from a single individual was diluted 20 times before undergoing polymerase chain reaction.

For experiment of half-life, cells were grown to ~50% confluency before RNA polymerase activity was blocked by 10μg ml−1 actinomycin D (sigma) in DMSO.

Cells were grown to 70% confluency before RNA polymerase activity was blocked with 10  μg/mL actinomycin D (Sigma) in DMSO.

By running backwards, a duplicate of the just-completed sequence is produced in an inverted orientation before the polymerase switches back to the correct template.