Before examination, patients completed a questionnaire reporting type and timing of dizziness symptoms and hearing status.
Before examination, cells were refreshed with complete medium and cultured for another 24 h.
Before examination, samples were transferred back into LN2.
Before examination of promoter function, WNT4 expression was confirmed in the primary cell lines.
Before examination, the dried samples were pressed into sheets with a hydraulic compressor.
Before examination, fecal samples are sterilized by heating for 12 hours at 70°C.
Before examination of the fibril-chaperone complex, the spectrum of αSyn fibrils in solution was explored.
Other samples were prepared as cross-sections using an epoxy resin and polished before examination.
The grids were allowed to dry for an additional 10 min before examination under the electron microscope.
Control oocytes were treated with the same concentration of DMSO in the medium before examination.
For electron microscopy, ultrathin sections were stained with uranyl acetate and lead citrate before examination.
