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Their response, or lack thereof, to androgen treatment for growth, as well as in reporter assays, was determined as a way to authenticate the PCa lines.
This observed phenomenon was comparable to the ER silenced MCF-7 cell line thus authenticating the presumed antiestrogenic activity of DIM which appears to utilize the estrogen receptor and its ability to modulate E2-ER system and signal transduction pathways which are E2 mediated such as the pAkt and ERK pathways, as described earlier by us and others [14], [47] [50].
Authenticating exit lines or last words has never been an easy challenge for historians.
We used DNA fingerprint analysis short tandem repeat (STR) profiling to authenticate the six ACC cell lines cited above, which include ACC2, ACC3 and ACCM.
To authenticate the identity of each cell line, short tandem repeat (STR) polymorphisms in microsatellites were analyzed in genomic DNA using the PowerPlex1.2 kit (Promega).
Short tandem repeat profiling of 14 and 15 loci, respectively, was used to authenticate the SKOV3 and TOV21G cell lines in 2012.
ATCC routinely authenticates these lines by isoenzyme (interspecies) and STR (intraspecies) analysis.
Short tandem repeat profiling is the standard method for authenticating cell lines.
GBSI itself is encouraging the research community to put in place accepted standards for authenticating cell lines.
Mantegna produced approximately 20 plates (only seven of which are completely authenticated), all line engravings in the broad manner.
Tools for authenticating cell lines are critical for quality control in cell-based biological experiments.
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