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The PCR program consisted in initial steps at 37°C for 10 min and 95°C for 15 min followed by 55 three-step cycles at 94°C for 30 s, AT°C for 40s and 72°C for 1 min and a final step at 72°C for 10 min. AT stands for the annealing temperature specific to each primer set [see Additional file 2: Table S1].
At stands and carts, they fueled up on everything from popcorn and caramel-filled churros to hamburgers, grilled meat and sandwiches.
At stands in the various markets, she tried to explain the differences among a dizzying variety of masa-based antojitos — a tlayuda and a garnacha and a memelita and a huarache and a tetela and even something called a gringa (an appellation that I told Abigail she should not take personally).
At Stands 4 and 5 there were some plots with very steep slopes of > 50% and sometimes > 100% (Table 1).
At stands with a stand age of 90 years, tree density, canopy height, mean diameter at breast height, basal area, and live tree biomass carbon stocks reach to nearly 430 tree ha-1, 27 m, 48 cm, 82 m2 ha-1 and 300 Mg C ha-1, respectively.
Street vendors hawk water and soft drinks at stands with half-empty refrigerators.
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I'm not great at stand-up.
At Stand Comedy Club, until 28 August.
At Stand Comedy Club until Sunday August 30.
Fig. 6 Predicted AGC at stand level.
Co-Founder and Director at Stand Agency.
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