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Asp at polymerase basic 2 protein (PB2) residue 701 was associated with reduced transmissibility.
The HBV population that emerged during the interruption of lamivudine treatment did not show mutations at polymerase positions 519, 526 and 550.
Other known virulence mutations, including at polymerase basic protein 2 position 627, were not present in any of the viruses characterized.
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The thermal profile for all analyses involved incubation for 2 mins at 50°C, polymerase activation for 10 mins at 95°C then PCR cycling for 40 cycles of 10 secs at 95°C dropping to 60°C sustained for 45 secs.
Although the RT alone and the RT/HBY 097 complex were crystallized in the presence of ATP, only the RT has an ATP coordinated with two Mn2+ at the polymerase active site.
At least polymerase η, Rev1 and to some degree polymerase κ have been related to SHM.
Pyrophosphorolysis has been useful in studying the events occurring at the polymerase active site.
Instead it is more likely that the 14/28 substrate assumes a conformation at the polymerase active site incompatible with pyrophosphorolysis.
RSC activity has been implicated in nucleosome repositioning and maintenance of the nucleosome free region (NFR) at RNA polymerase II (Pol II -transcribed promoters [27], [28].
Evidence suggested that the recombination site occurred at the polymerase and capsid junction on ORF1.
At present, polymerase chain reaction (PCR) is the dominant method to detect nucleic acid sequences.
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