Sentence examples for At pcr from inspiring English sources

PCR of the cDNA was performed using Platinium High Fidelity Taq using the following pairs of primers: PDE5 sense: (5'-3') ACC GCT ATT CCC TGT TCC TT PDE5 antisense: (5'-3') AAG GTC AAG CAG CAC CTG AT PCR parameters were: 35 cycles (94°C for 45s, 58°C for 45s, and 72°C for 1 min).

At PCR, portraits are hung alongside landscapes and huge abstractions (the traces of dust on processing paper) take their place beside equally vast studies of ordinary things, like a weed standing tall in his unkempt garden.

Past attempts at PCR for ocular TB have yielded low positivity rates ranging from 33.3% for retinal vasculitis to 66.6% for panuveitis [4].

Despite multiple attempts at PCR amplification and cloning, a number of ORFs were not amplified and/or captured in the donor vector.

Two attempts at PCR validation was attempted for each genome.

Three herds (37.5%) resulted positive for B. caballi at PCR.

a AT-PCR annealing temperature (° C).

Further discussion of these is beyond the scope of this article; however, we direct the reader to a study by Acinas et al. [ 25], who looked at PCR-induced artefacts in sequencing library construction.

Labor time associated with next-generation library preparation and target enrichment for UCEs for 90 taxa (~3 weeks for one full-time person) is roughly the same as for a single attempt at PCR-amplification and cycle sequencing of 10 individual genes, if not less.

Samples were than kept at −20 °C until PCR at the home based laboratory.

Rabgef1 is specifically expressed in the erythroleukaemias at 25 PCR cycles (lane a) but was amplified in all samples at 28 PCR cycles (lane b).