The rate of Tn dissociation in the non-overlap region was 200-fold faster at pCa 4 (C-state region) than at pCa 9 (B-state region).
When Tn contained engineered TnC mutants with weakened regulatory TnI interactions, the apparent exchange rate at pCa 4 in the non-overlap region increased proportionately with TnI TnC regulatory affinity.
At pCa 9, the rate of Tn dissociation in the overlap region (M-state region) was 100-fold faster than the non-overlap region (B-state region) suggesting that strong cross-bridges increase the rate of Tn dissociation.
At pCa 4, the rate of Tn dissociation was twofold faster in the non-overlap region (C-state region) than the overlap region (M-state region) that likely involved a strong cross-bridge influence on TnT's interaction with actin-Tm.
"Concrete homes and safe rooms are a proven way to keep people safe during a high wind storm," said Donn Thompson, senior director of market development at PCA. "These resilient buildings not only provide safety, but peace of mind when the next natural disaster strikes".
Stretch activation can be induced in skinned fibers at pCa <6.0 [30].
Normalized force was calculated as the ratio of the measured force at a given pCa and the maximally activated force (i.e., force at pCa = 4.33).
The force of contractions generated at 10−7 M free calcium concentration were recorded and expressed as a percentage of maximal contraction at pCa 5.
IFM also produces sustained isometric contractions at pCa <4.5, which we refer to as isometric high static tension or iso-HST, that correspond to an isometric tetanus in vertebrate muscle.
