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Mitotic Centromere-Associated Kinesin (MCAK) controls MT dynamics at MT plus-end [46], [47] and is thought to participate in the correction pathway for erroneous KT-MT interactions by promoting MT turnover at KT [48], [49].
"I think we should have a choice: They should teach you both," said Meagan Hass, 14, while eating pizza after school at KT's restaurant with her friend Abbi Hake.
At the receiver side, the signal in (1) should be matched filtered and the transmitted symbols should be regenerated by sampling r(t) at (kT - hat tau ) time instants, where (hat tau ) is timing delay estimation provided by synchronizer.
Hepatoma Up-Regulated Protein (HURP) is a MT associated protein that is involved in K-fiber stabilization at KT [43] [45].
Given the fundamental role of Hec1 in MT interaction at KT we decided to investigate the nature of KT-MT attachments in EGFP-Hec1 expressing cells.
Accordingly, CENP-E antibody intensity at KT was definitively lower in late prometaphases/metaphases relative to early prometaphases in vector-transfected cultures (Figure 7A).
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If you are at the convention stop by Booth #112 and look at KT-Tech's product for me.
The database of measured data comes from a special lipids database developed in-house (SPEED Lipids database at KT-consortium, DTU, Denmark).
In EGFP-Hec1 expressing cells more mitoses showed MCAK both at poles and KTs and several EGFP-Hec1 cells, but no vector transfected cell, showed MCAK only at KTs (Figure 6B, EGFP-Hec1 and graph).
These results clearly show that MCAK re-localizes from centrosomes to centromeres upon EGFP-Hec1 expression, in line with previous reports showing that erroneous KT-MT interactions i.e. merotelic attachments recruit MCAK at KTs [49].
On the contrary, EGFP-Hec1 KTs both in prometaphases with alignment defects and in disorganized prometaphases accumulated CENP-E at levels that were intermediate between those of early and late prometaphases (Figure 7A), suggesting that persisting initial interactions driven by the EGFP-Hec1 protein retained CENP-E at KTs.
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