The median age at IBD diagnosis was 13.7 years (range: 1.5 18).
However, looking at IBD subtypes, this association was only significant with CD.
Furthermore, the survey was based on patients participating at IBD workshops organized by a self-help association but open for public.
Importantly, binding sites for HNF4a were significantly enriched at IBD associated SNPs that lay close by an LPL TEM upregulated gene, but not at IBD associated SNPs that do not map to upregulated genes (p=0.01, see online supplementary table S1).
The first limitation is selection bias: the study was conducted at IBD specialist clinics in urban areas and subjects may have been well-educated regarding their disease and treatment.
Genomic IBD was obtained by calculating the allele frequencies of the founder alleles at the IBD markers, i.e. f ij for founder allele j at IBD marker i.
The amount of hydrogen evolved during the oxidation of formaldehyde at Ib electrodes was determined as a function of the potential.
The patients were staged according to the tumor, node, metastasis (TNM) Classification of Malignant Tumors, 7th edition (International Union Against Cancer, 2009), with 7 cases staged at IA, 9 at stage IB, 17 at stage IIA, 43 at stage IIB, 27 at stage IIIA, 21 at stage IIIB, and 3 at stage IIIB.
According to the staging of the International Federation of Gynecology and Obstetrics (FIGO) in 2009, there were 61 cases at stage I (38 cases at stage Ia and 23 cases at stage Ib), 7 at stage II, 28 at stage III, and 6 at stage IV.
HE4 expression in endometrial cancer was 76.32% at stage Ia, 91.30% at stage Ib, 71.43% at stage II, 96.43% at stage III, and 100.00% at stage IV, respectively, which showed an increasing tendency with increased clinical stage.
The strongly positive expression rate of HE4 in endometrial cancer was 31.57% at stage Ia, 56.52% at stage Ib, 28.57% at stage II, 71.42% at stage III, and 83.33% at stage IV, respectively, which showed an increasing tendency with increased clinical stage (PIa III = 0.001, PIa IV = 0.016, PI III = 0.008, PI IV = 0.047, PII:III = 0.035, PII:IV = 0.048, and P(I+II):(III+IV) = 0.001).
