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Collected samples were sealed in a polystyrene plastic bag (Ziploc bag), labeled on a card (OIE, 2008) designed for this purpose and the securely transported under refrigeration to the laboratory for storing at freezer temperature (−20 ° C) till analysis.
The major constituent of the S. hortensis essential oil such as Carvacrol was increased in all three storage temperatures, so that the highest and the lowest increasing occurred at freezer and room temperatures, respectively.
The Ssp4 variant with Asp at residue 36 was also shown to facilitate exceptional spore survival at freezer and refrigerator temperatures.
During collection each container was labeled with donor ID and hospital registration number and kept at freezer (below −20°C).
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She complained that while working at the freezer department at a Sam's Club in Concord, Calif., several men she had trained were promoted over her.
The museum has guards at the freezer's entrance and exit.
The culture vial was stored in the YPD medium containing 30% of glycerol at −80 °C freezer.
To assess long term stability, six plasma replicates of LQC and HQC stored at deep freezer (30 days, − 40 °C) and standard-spiked-plasma sample were used.
Serum samples were stored at −70°C freezer.
The plasma was immediately frozen at -80°C freezer.
After centrifuging and flash freezing, cell pellets were stored at −80°C freezer until use.
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