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The algorithm employed kept track of multiple chained propagations and only halted at deletions [21].
First, we looked at deletions on chromosome 16p11.2 (28.4–31.0 Mb) of at least 593 kb in both of our GWAS samples (21).
In principle, any gene would be suitable, however, as we were expecting to look at deletions, amplifications and rearrangements, we chose a gene that was unlikely to be involved in any of these processes.
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To verify conservation of function, bryP was introduced into AT-deletion mutant strains of a heterologous host containing a PKS cluster with similar architecture, and polyketide production was partially rescued.
Numbers in red at deletion decision points indicate the number of isolates at that stage for that reason (Fig. 1).
Brachypodium and sorghum PSY3 sequences are not characterized by such repeated motifs at deletion breakpoint, suggesting that intron loss occurred by simple genomic deletion.
The deletion of two nucleotides in RNAP gene truncates the primary translation due to early stop codon, while a second translational peptide starting from GTG just at deletion point can remediate the polymerase activity.
These nine loci are: duplications at 1q21.1, the WBS region, the AS/PWS region, at VIPR2, at 16p13.11 and deletions at 3q29, distal 16p11.2, 17q12 and 17p12.
Similarly, an AT-genome deletion and DT-genome duplication suggested a DT-biased conversion.
He describes himself as "angry at the deletions and astonished by the process".
The great Blue Note jazz catalogue is shrinking further with the announcement of a second wave of deletions at year's end.
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