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The question whether synapses clustered at DCC were of different strengths than other synapses was addressed by measuring presynaptic secretion levels using the synaptic vesicle recycling indicator, FM1-43.
Follow-up calls are made by the data manager at DCC to verify data that are missing or contradictory.
Three out of the five evaluable recurrent TCCs exhibited allelic loss at DCC, two of which were superficial.
Allelic loss was observed at DCC in 8/24 (33%) samples, associated with muscle-invasive disease (P = 0.05).
The other major challenge the data management team at DCC faced was the use of different language CRFs in some countries.
Homozygous deletions at DCC, DSG2 and DSC3 as well as copy number changes of the HDAC9, TWIST1 and ITGB8 region were validated by real time PCR for the 24 tumors screened by SNP array.
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Hence, synaptic density is enhanced at DCCs compared to other areas along the dendrites.
As mentioned in the Introduction, the high dendritic proximity found at DCCs likely facilitates ephaptic coupling among the converging dendrites.
At DCCs, dendritic branches are associated with higher synaptic density and strength than at other regions along the branches.
We noted that the density of intersections axons make with dendrites at DCCs is on average 2.5-fold higher than outside the DCCs (Figure 6g).
2. Clustering of synaptic connections at DCCs results in local reduction of inter-synaptic distances, as compared to those existing outside DCCs.
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