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To assess rigorously the potential under-annotation of short ORFs with homology, we exhaustively compared the polyORFome all possible ORFs in 64 prokaryotes (53 bacteria and 11 archaea) plus budding yeast to itself and to all known proteins.
Given the small sample size at each participating SPH we could not assess rigorously the validity or reliability of the tool, but future studies with larger samples could usefully contribute to this.
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The effect of model uncertainty could be assessed rigorously since all bias and variance estimates were derived for specific models and for different test and training partitions.
Accordingly, if noninferiority trials are to be applied to clinical and regulatory decisions regarding the marketing and use of new treatments, the assumptions must be made explicit and their influence on the resultant conclusions must be assessed rigorously.
Since KIEs may impact isotopic data and ultimately jeopardize the validity of the biological interpretations, their impact must be assessed rigorously.
However, in light of the available data showing that stopping DMARDs is associated with an increased flare frequency, 108 110 the committee felt that tapering should be performed cautiously and assessed rigorously.
These clones, along with those identified from the genome-wide analysis, were then assessed rigorously in order to exclude any that might not be representative of the genome (e.g. chimaeric ligation or the result of rearrangement in E. coli).
However, a recent review of curricular innovations in undergraduate science courses (both lecture and laboratory) suggests that teaching practices in undergraduate science and engineering courses are changing in the direction of inquiry but, unfortunately, are still not necessarily being assessed rigorously (Ruiz-Primo et al., 2011).
15 19 One area of research where fidelity of delivery has been assessed rigorously, using a reliable method of specifying intervention content in terms of behaviour change techniques (BCTs), is behavioural support for smoking cessation 14 15 and an intervention to increase physical activity among sedentary adults.
However, the retrospective nature of the TCGA project and its use of tumors not necessarily linked to specific clinical trials, in which the outcome of therapy can be assessed rigorously, limit the ability of the TCGA to establish whether the genomic data can 'predict' the action of the current treatment standards (that is, anti-estrogens or chemotherapy).
This is particularly difficult when schools are being assessed very rigorously on how many A*-C students they have.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com