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All the reagents were endotoxin-free as determined by the Limulus amoebocyte lysate assay (sensitivity limit, 0.1 ng/ml).
No solution contained detectable LPS, as determined by the Limulus amoebocyte lyase assay (sensitivity limit 12 pg/ml; Biowhittaker Inc, Walkersville, MD, USA).
The IL-17A or IL-27 contained less than 1.0 U endotoxin/1 μg cytokine, as determined by Limulus amoebocyte lysate assay (sensitivity limit 12 pg/ml; Biowhittaker, Inc., Walkersville, MD).
The assay sensitivity limit, defined as the concentration of TFF3 that can be distinguished from 0, was ∼5 ng ml−1 of recombinant TFF3 and detection was linear over a range of 20 1250 ng ml−1 (r=0.99).
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The assay sensitivity limits were 7 pg/mL for IL-4, and 30 pg/mL for IFN-γ and IL-10.
Along with parameters commonly provided by manufacturers (e.g., assay sensitivity, lower limit of detection, and assay precision determined by reproducibility of replicate measurements), immunoassay utility should be evaluated also by matrix-specific fold change in cytokine concentration reliably detectable by multiple comparisons methods.
In contrast to previous studies, the simulation results suggest that prevalence estimates based on PCR genotyping assay results generally overestimate the true infection burden; genotyping assay sensitivity has limited effect on the composite prevalence estimates; and the decline in specificity is more influential.
The sensitivity of the used assay takes the dilution of the samples into consideration and is calculated according to the formula: Assay sensitivity = Analytical limit of detection x sample dilution = 0.033 ng/ml×3 = 0.1 ng/ml.
The differences in response that were seen may have resulted from subtle assay-related differences (e.g., cellular background, gene source, expression level, or assay sensitivity at the limit of quantification), suggesting that these mutant forms are severely impaired with a very limited capacity for pharmacological chaperone rescue.
A nuclease P1-enhanced 32P-postlabeling assay, having a sensitivity limit of 1 adduct in 10 9-10) DNA nucleotides, was found suitable for measuring aromatic DNA adducts derived in vitro from catechol, benzenetriol (BT), phenol, hydroquinone (HQ), and benzoquinone (BQ), potential metabolites of benzene.
Taking normal plasma levels of E1 and E2 into account, assays with a sensitivity limit of 5 7 and 1 2 pM respectively is needed to detect >90% suppression of plasma hormone levels during treatment with an aromatase inhibitor.
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