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ELISPOT data were first processed by expressing each well as SFC per million PBMCs as per validation of ELISPOT assay [ 18, 19], followed by subtracting the mean spot number of the triplicate of unstimulated cells from that of the test triplicate.
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Second, the diagnostic performance of iFR was evaluated for a 0.75 ischemic FFR cutoff, as per original validation studies and DEFER FFRR or iFR ≤0.75 as a reference test to define significant stenoses).
Drinking on the PNQ was assessed in two ways: 1) by asking the amount and frequency of drinking during this pregnancy; and, 2) by comparing time of last drink with normal menstrual cycle as per the validation of the SAQ [ 22].
The cost is $10.00 per validation ticket.
The reagent cost per validation is estimated to be $20 (USD) per variant on average.
The model we developed performed as per our expectation and gave good cross-validation prediction accuracy.
As per the cross validation results in Table 2, this supports the possibility that blending datasets where the context of the cyber hate could contain multiple types of hate speech due to intersectionality (in this case, homophobic and racist) will improve classification results.
The method used was developed and validated as per ICH (Q2-R1) guidelines for validation of analytical methods [34].
The optimized ion chromatography method was validated as per the ICH guidelines and validation of compendial procedures as per USP general chapter 1225.
IL12 and CXCL9 levels were not considered for these experiments as per Invitrogen's assay validation data on EDTA plasma samples.
To investigate the gut microbiota from metabolic phenotypes we first performed a DGGE on subsets of three mice per group as a primary validation of our hypothesis.
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