The vertical dashed lines indicate the high-speed flow burst events larger than V⊥x = 300 km/s, as labeled "1" through "13" above the flow panels.
(A ) Coomassie stained SDS-PAGE gel of purified, recombinant CPAP937 1124 and its mutants (as labelled in colours above the gel).
To probe the interaction between Aβ and ApoE CT; Aβ was labeled as above and ApoE CT was labeled using 3H1, directly conjugated to Cy3 [44].
Cells were fixed and immunofluorescently labeled as above, using an anti-H3S10ph antibody to identify mitotic cells (Hendzel et al. 1997).
To identify the chromosome(s) holding the transgenic BAC insertion in the rat lines the BAC clone was labeled as above and co-hybridized with a panel of rat M-FISH probes (22Xrat mFISH probe kit by MetaSystems).
For IF microscopy, cells in 24‐well dishes were washed with PBS followed by fixation with 4% paraformaldehyde for 10 min. Following PBS washes, cells were labeled as above for tissue sections and image obtained with an Evos fluorescent microscope (AMG).
As a reference probe universal human reference RNA (UHR; Stratagene) was amplified and labeled as above.
Cilia are labeled as above.
NIH cells were transfected and labelled as above and stimulated with FCS.
Culture supernatant proteins were precipitated with 9 vol of trichloroacetic acid:acetone (1∶8 v/v for 2 h at −20°C) and then analyzed by SDS-PAGE and immunoblotting as described above (labeled as "extracellular proteins" in Fig. 3A).
