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It does highlight that even a relatively weak inhibitor as assessed previously by chemotactic assays can still counteract the effects of a strong activator.
All tumors expressed high levels of S100A4, as assessed previously by immunohistochemistry, and within each stage group, one tumor harboring wt and one mutated TP53 were selected [ 10].
The rats performed various tests including mobility, stance, and gait in the open field, gait on a rotarod at steady and accelerating speed, swim speed in a narrow pool, memory in a modified water maze test as assessed previously [ 19, 20, 22], and gait on a modified ladder test [ 38].
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The concentration and purity of RNA as well as DNA synthesis were assessed as previously described [ 31].
Histology and contusion volume were assessed as previously described12.
In addition, clinical improvement was assessed as previously reported (Hanifin & Rajka, 1980; Kimata et al, 2002).
The quantity of formed biofilms was assessed as previously described with some modification46,47.
Reversible protein oxidation was assessed as previously described using the PEG-switch assay48.
Glucosidase inhibitory activity was assessed as previously described (Sancheti et al.2011) with minor modifications.
Cell viability, and morphology were assessed as previously described [31].
Lung function was assessed as previously described [11] with modifications.
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