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As analysis before, the peaks of envelope ACF tend to appear at the pitch period.
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We performed a t-test analysis as before.
Cell surface expression and receptor-binding of each altered EnvB protein was confirmed by flow cytometric analysis as before.
The precipitate collected after 3 days in serum-free conditions was then washed in DMEM and subjected to SDS-PAGE analysis as before.
Cells were exposed to nanoparticles for up to 24 h and processed for image analysis as before.
For this analysis, as before, we validated that K = 2 clusters best explains the data (data not shown).
Cells were collected at 45 minutes, 2 h and 8 h upon osmostress and RNAs were subjected to microarray analysis as before.
To test whether Arfrp1 or μ1-adaptin interact with Frizzled-6 or Celsr1, we performed GST-pull down analysis as before.
This approach allows us to report multiple overlap alignments (including split alignments) for an experimental map while using the q-value analysis, as before, to report all alignments with q≤0.01.
Plasma was separated from red blood cells by centrifugation (4,000 rpm for 10 min) and then stored at −20°C before As analysis.
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