Bacteria were grown on Brain Heart Infusion (BHI) based agar plates supplemented with 5% horse blood and when appropriate, with erythromycin (10 µg/mL) or streptomycin (10 µg/mL) in an atmosphere containing 5% CO2.
Dissected guts were then put into the fixative for 20 minutes before staining with 4′,6-diamidino-2-phenylindole (DAPI) and (when appropriate) with Sytox green in PBS at room temperature for 20 minutes.
Conducted by Robert Bass, the Collegiate Chorale sounded very good: singing with a focused roar when appropriate, with a lovely warmth in softer passages, and everywhere with a discipline of articulation and phrasing unusual to a large group hovering somewhere between the professional and the amateur.
A repeated measures two-way ANOVA was performed when appropriate with a Bonferroni post hoc test.
One-way analysis of variance (ANOVA), Newman-Keul, Kruskal-Wallis, or Holm-Sidak multiple comparison tests were performed when appropriate, with a 0.05 alpha level unless indicated differently above.
The resulting log reductions were subjected to a single t-Test and, when appropriate, an ANOVA analysis with a Tukey test to generate statistical significance and grouping across samples, materials, and strain types.
For discrete risk factors, odds ratios (OR) were calculated and when appropriate, a pooled OR with 95% confidence intervals (95% CI) was calculated.
By grade 12, students should be able to do the following: Formulate a question that can be investigated within the scope of the classroom, school laboratory, or field with available resources and, when appropriate, frame a hypothesis (that is, a possible explanation that predicts a particular and stable outcome) based on a model or theory.
Expeditious treatment of the underlying infection is performed medically with antibiotics and when appropriate surgically with drains or re-exploration (see Figure 1C).
Baseline, procedural, outcome and validity data were systematically appraised and pooled, when appropriate, with random-effect methods.
In all cases, performance is investigated by simulation and compared, when appropriate, with asymptotic bounds; these latter are usually met after a moderate number of MA dwells.
