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Activity of TRAIL and TRAIL variants was restored by stepwise dilution with refolding buffer.
So far, no correlation between TRAIL resistance and TRAIL decoy receptor gene expression has been reported.
TRAIL R1 (death receptor 4) and TRAIL R2 (death receptor 5) induce apoptotic cell death.
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Thus, there is no straightforward correlation between the levels of TRAIL-R1 and TRAIL-R2 and TRAIL-receptor preference in TRAIL-induced apoptosis.
As shown by flow cytometry, TRAIL-R1 and TRAIL-R2 were expressed constitutively on higher-proliferating RASFs in vitro, whereas TRAIL-R3 and TRAIL-R4 were not detectable.
Having established the specificity of our TRAIL-R1 (and TRAIL-R2) antibodies in TRAIL-R1 (and TRAIL-R2) transfected cells, cytoplasmic staining prevailed also in this setting [ 22].
Both endogenous and TRAIL-induced NF-κB activity was drastically reduced after infection with AdIKKβKA.
Besides TNF α, hyperthermia can also promote Fas ligand and TRAIL-induced apoptosis.
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