They arrested this mps1 mutant after duplication of spindle pole bodies (SPBs; microtubule-organizing centers in yeast) but before DNA replication and SPB separation, by using a cdc34-2 temperature-sensitive (ts) mutant.
Our localization analysis of wild-type proteins provides a dynamic picture of the process of SPB duplication that can be used in the future for the study of cell cycle and SPB mutants.
At least two models could explain this suppression: first, pom152 ∆ might act as a "dosage suppressor" by freeing factors from the NPC that are involved in both NPC and SPB assembly, and second, reduced NPC assembly in pom152 ∆ may indirectly result in NE changes that allow SPB duplication in the absence of MPS3 (Jaspersen and Ghosh 2012).
As described in "EFD" section, two types of digital data, DPB and SPB, are generated in the EFD.
In addition, field tests such as noise emissions by CPX and SPB methods, texture depth, friction coefficient and in-situ water permeability were performed.
By incorporating a range of forest and SPB dynamics over long time scales, our results extend previous modeling studies, and inform forest managers and policy-makers about the potential future effects of SPB.
To examine the efficacy of cost sharing in promoting participation, a survey was sent to 1200 NIPF landowners in Virginia, where southern pine is prevalent and SPB is a concern.
The original request for proposals for a heavy escort fighter (Tyazholyy Istrebitel' Soprovozhdeniya) was received at the Polikarpov OKB in November 1938, but the press of work with the I-180 and SPB prototypes prevented any significant design work until the third quarter of 1940.
Uch2p-mCherry and Pcp1p-mCherry were used as NE and SPB markers.
CVB and SPP had low pairwise divergence (1%, 0.3 1.0 Mya, Table 4) and so did BB and SPB (1%, 0.4 0.7 Mya, Table 4).
Assays regarding protein levels, Tub4 complex assembly and SPB binding gave first insights into the defects of the phospho-mimicking Tub4 mutant proteins.
