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In fact, the collection, which looks as folksy as if it had been painted by Grant Wood, is the second act of a family that once manufactured vintage-looking jeans for labels like Levi's, Ernest Sewn, Rogan and RRL.
The main resistance mechanisms are mutations in the erm(41) and rrl genes.
Mutations in the erm(41) and rrl detected by DGGE were 100% identical to mutations detected by DNA sequencing.
Here we report PCR-based denaturing gradient gel electrophoresis (DGGE) as an alternative method for rapidly detection of mutations in erm(41) and rrl among M. abscessus isolates.
This is the first report to identify PCR-based DGGE as a practical, relatively inexpensive technique for rapidly detecting mutations in the erm(41) and rrl genes associated with clarithromycin resistance in M. abscessus complex.
Details concerning DNA extraction, preparation of DNA pools and RRL libraries have been described previously [24].
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RRL-F and RRL-O complexes showed predominantly activity-dependent elution.
In contrast, at 100 nM compared to 30 nM DNA concentration, 293T-O TERT complexeshoweded no additional DNA binding and RRL-F and RRL-O complexes showed only limited additional association with DNA.
There is excellent correlation of two-color co-localization to two-step photobleaching results for 293T-O, RRL-F, and RRL-O but not 293T-F.
TERT monomer complexes exceeded TERT dimer complexes in the DNA-bound 293T-O, RRL-F, and RRL-O populations across almost the entire range of modeled labeling efficiencies.
Comparison between the pair of 293T or RRL purifications suggests that most of the TERT in 293T-F and RRL-F was not assembled as telomerase RNP.
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