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MGB probes specific for type 2b were labeled with FAM in both type 2a/2b and 2b/2c assays, and MGB probes specific for type 2a (type 2a/2b assay) and type 2c (type 2b/2c assay) were labeled with VIC.
RS, MH, HL and MGB are postdoctoral research fellows at the Retinal Imaging Research and Reading Center.
Singleplex qPCR primers and MGB probes were designed individually for the detection of the three targeted viroids, and subsequently combined in a one-step multiplex RT-qPCR reaction.
Both PriProET and MGB assays are capable of detecting 20 copies of a DNA standard per reaction and are linear from 2 × 108 to 2 × 102 copies/μl.
Some degree of tonotopy was identified in the IC, SOC, and MGB in the pooled dataset though it could not be assessed per subject due to a lack of statistical power.
Not having the range to reach St Nazaire unaided, the MTB and MGB were taken under tow by the Campbeltown and Atherstone.
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MGB-353 and MGB-354 have MIC99s of 3.1 μM against the mycobacterium M. tuberculosis.
In order to verify the RT PCR results, Southern blot was performed for CK-19 and MGB-2.
In their study, prostate-specific antigen (PSA), carcinoembryonic antigen (CEA), and MGB-1 and -2 were highly expressed in the primary tumours.
Predesigned gene-specific primers, 18SrRNA internal control primers and MGB-FAM-labeled probes were purchased from Applied Biosystems (Carlsbad, CA, USA).
Hybridisation was performed in Rapid-hyb buffer (Amershampharmacia biotech, Piscataway, NJ, USA) with oligonucleotide probes (CK-19P and MGB-2P) previously biotin labelled by biotin N-ddATP (10 pmol ml−1, NEN Life Science Products Inc., Boston, MA, USA).
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