The second tail fiber gene in cluster 5 presented two distinct alleles; phages FSL SP-031, FSL SP-038 and FSL SP-049 share one conserved tail fiber (99 to 100% aa identity) as do phages Se2, SEPT3, SS3e, and FSL SP-101 (94 to 95% aa identity), with only 27% average aa identity between these two tail fibers (see Additional file 6).
In addition to 88 L. seeligeri specific ORFs (i.e., ORFs only found in both L. seeligeri FSL N1-067 and FSL S4-171), whincludeduded three ORFs encoding specific internalin-like genes, we also identified strain specific ORFs (Table 3), including seven and three genes that encode putative internalins (in FSL S4-171 and FSL N1-067, respectively).
In two of the Paenibacillus genomes (FSL H7-689 and FSL R5-192) we identified an operon annotated as encoding the lantibiotic mersacidin biosynthesis protein LanM and the corresponding transporter protein (Table 3).
Phages FSL SP-058 and FSL SP-076 share a conserved backbone, but show considerable variation in the two tailspike encoding genes, which, as discussed below in detail, is often related with host range variability [ 30].
Among the 16 genes, two were selected from the RSL, one from both the RSL and FSL, and 13 from the FSL.
This analysis identified additional putative bacteriocins of class I (i.e., lanthipeptide, lassopeptide, and sactipeptide bacteriocins) in four genomes (FSL H7-687, FSL R7-277, FSL R7-269, and FSL R5-192), as well as two class II bacteriocins (i.e., UvB and un-classified class II bacteriocin) in two genomes (FSL R5-860 and FSL H8-237) (Table 3).
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HBD-2 induction was dramatically upregulated in the presence of SB216763 and FSL-1.
Inactivated S. aureus and FSL-1 showed a similar protective effect (Fig. 3B).
Purified TLR agonist controls, LPS and FSL-1, had optimal and maximal stimulation at 6 hours (Figure 1B).
Purified LTA from S. aureus, lipomannan and FSL-1 was obtained from Invivogen (San Diego, CA, USA).
