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The genes differentially expressed in each group were saved as lists referred to as SRCB, CUCB, and ESCB gene lists for convenience in further analysis.
The numbers of probe sets specifically expressed in each of the SECNT embryo groups were as follows: CUCB, 482; SRCB, 449; and ESCB, 176.
The analysis clearly showed that the gene expression profiles of the SECNT embryos (71 samples) were invariably clustered into 3 groups corresponding to the type of donor cell used: the CUCB, SRCB, and ESCB groups.
The results of the analysis showed that a relatively large number of probe sets were significantly differentially expressed between the controls and the 3 SECNT embryo groups: 1,150 (upregulated 531; downregulated 619 [53.83%]), 1,075 (upregulated 565; downregulated 510 [47.44%]), and 609 (upregulated 180; downregulated 429 [70.44%]), in CUCB, SRCB, and ESCB groups, respectively (Figure S1).
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These profiles were clearly distinguishable from those of CUCBs and ESCBs as well as other SRCBs.
Table 1 shows the average of ESCB and the convergence speed of two methods for 50 frames.
Table 1 demonstrates the average of ESCB and the convergence speed of two methods for 41 frames.
Table 1 illustrates the average of ESCB and the convergence speed of two methods for 66 frames.
Using microarray data, we were able to identify a particular set of genes that were inappropriately reprogrammed in each SECNT embryo group: 31 genes in CUCBs, 46 genes in SRCBs, and 11 in ESCBs (Table 2).
The model has been used in the Working Group on Econometric Modelling (WGEM) of the European System of Central Banks (ESCB) to simulate various fiscal consolidation measures for Spain (see Stähler and Thomas 2012).
Of the 154 genes that were involoved in regulation of biological process in ESCBs, 12 and 7 genes were expressed at low and high levels, respectively, in ES cells at threefold or more (Table S3).
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