An extract portion containing 30 μg of soluble protein in 2 × Laemmli buffer (Sigma) was heated for 10 min at 90 °C and loaded onto a 4 12%% (w/v) NuPAGE Novex Bis-Tris gel (Life Technologies) for separation by 1D SDS-PAGE electrophoresis using 2- N-morpholino -ethanesulfonic acid (MES) buffer alongside Novex SeeBlue Plus2- N-morpholino -ethanesulfonicstandards.
For example, assuming that has a duration greater than 500 ms, is the extracted portion of the waveform from 300 to 500 ms.
Recently, we proposed a new isosurface extraction algorithm that extracts portions of the isosurface in a view-dependent manner by ray casting and propagation.
Artists will extract portions of the story, tidy them up, set them to pop or hip hop or classical music, then allow radio stations to play it to death.
At one point, Mitchell wrote in an 1896 article for the British Medical Journal, he took a fair portion of an extract made from mescal buttons and followed it up with an additional dose.
For the ortholog groups used for molecular evolution analyses, we then extracted the portion of the alignments with representation from all taxa.
We segmented the cryoEM density and extracted a portion that represents a hexamer formed by two layers of protein subunits using the segmentation tools in UCSF Chimera.
To detect orthologs of intronic snoRNAs, we extracted the portion of each intron alignment that corresponded to a snoRNA annotation in S. cerevisiae (SGD).
Using the amino acid alignments between the translated ESTs and the A. thaliana homologues, we identified and extracted that portion of each EST sequence representing the reading frame (these generally did not include the start or termination codons).
After 12 hr, cell DNA was extracted, a portion was sheared and tested for its content of RSV DNA (Fig. 6A), and the remainder was subjected to network formation.
After aligning the sequences of each triplet with Clustal W 2.0 [ 114], we extracted the portion of the alignments containing all three orthologous sequences and trimmed partial codons at the 5' and 3' ends (based on the A. thaliana sequence).
