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This observation is consistent with the well-known capacity of Rep78 and/or Rep68 to locally amplify the AAVS1 region due to the usage of its RBE and trs as an origin of replication [33].
According to the obtained experimental data, the usage of GNPs modified by specific oligonucleotides can amplify the sensor response of the SPR DNA hybridization sensor in ~1200 times.
We used a nested RT-PCR with a sensitivity of 2.5 copies/ml to amplify a region spanning the V3 env region and thus determine the coreceptor usage of the residual viruses in the plasma.
Alternatively, the primers used for those pilot experiments were less optimal, as usage of primers from a most conserved region (figure 3) amplified the head-to-tail sequences shown in figure 5.
Roc-COR-PK (WT, R1441C, N1437S and T1348N) constructs (aa1330-2147) were amplified from the corresponding full-length LRRK2 Vector and introduced in pcDNATM3.1/myc-His vector (Invitrogen) by usage of BamHI and XhoI.
Through the combinatorial usage of three primers with a single reaction set, two fragments of 290 and 159 bp were amplified from the cattle meat DNA, whereas only a fragment of 290 bp was obtained from the yak meat DNA.
Usage of chlorine skyrocketed.
Note my usage of the plural, surveys.
That is entirely constructive usage of technology.
No other usage of integrity here applies.
"The usage that's coming is the usage of its time".
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Since I tried Ludwig back in 2017, I have been constantly using it in both editing and translation. Ever since, I suggest it to my translators at ProSciEditing.
Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com