Exact(60)
All tissue samples stained anti-tPA positive.
All tissue rostral to the decerebration and all remaining cortex dorsal to the colliculi were removed.
All tissue sections underwent heat-induced antigen retrieval before staining with antibodies.
All tissue samples were stored at −80 °C for subsequent biochemical analysis.
All tissue and fluid was exhaustively evaluated.
All tissue sections were counterstained with haematoxylin.
All tissue densities are estimated at 1 g/mL [19].
All tissue culture reagents were purchased from Invitrogen.
All tissue sections were counterstained with hematoxylin.
All tissue donors provided written informed consent.
All tissue culture reagents were obtained from Invitrogen (Singapore).
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