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All fragments are assumed to be of spherical shape.
All fragments healed with an average intra-articular step of 3.0 mm (0.5 11).
All fragments were amplified with oligomers having uracil incorporated, using the Phusion U polymerase (Thermo Scientific).
All fragments were finally separated on a 2% agarose gel electrophoresis and visualized by ethidium bromide.
All fragments were cloned into the pGL3 basic backbone (Promega) digested with the appropriate restriction enzymes.
All fragments were then gel purified using the QIAquick Gel Extraction Kit (Qiagen).
All fragments were gel purified, then ligated using T4 DNA Ligase (Promega).
All fragments sequences are posted on the Data FTP page of Nematode.net.net
All fragments were amplified using proof reading polymerase (LA Taq, Takara) from C. elegans genomic DNA.
All fragments were derived from either glucose-6-phosphate or proteinogenic amino acids [21].
All fragments were monitored at each visit for visible signs of stress or disease.
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