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All currents and voltages were registered and controlled using a HEKA EPC10 amplifier.
All currents were recorded in the inside-out configuration with an Axopatch 200 amplifier (Molecular Devices, Sunnyvale, CA).
All currents were normalized to the cell capacitance calculated by Membrane Test (OUT O) in pClamp8.0.
All currents followed a Hodgkin-Huxley kinetic scheme (Hodgkin & Huxley, 1952).
All currents were recorded in excised patches at room temperature (22 25°C).
All currents for the activity testing were normalized to the highest acetylcholine dose applied precompound testing.
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