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Alignment criteria comprised a minimum of 90% of the query sequence mapped on the genome and the percentage identity of the sequence relative to the mapped segment greater than 95%.
We applied a robust and direct approach45,46 allowing the characterization of genome duplications by aligning the available genes (36,376) on themselves with stringent alignment criteria and statistical validation.
Given millions of sequences, the goal is to identify all pairs of sequences that are highly similar (or "homologous") on the basis of alignment criteria.
Poses are judged by detailed alignment criteria for a total of 80 points, plus ineffables like "grace" are considered.
We used equal or higher than 98% identity and 70% exon length coverage as alignment criteria to determine the match of an EST with a particular exon sequence.
These alignment criteria were chosen to maximize the likelihood of achieving unambiguous alignments while also allowing for potential gene family members, duplications, and limitations of the bovine genome assembly (i.e., assembly errors, chrUn unassigned sequence contigs).
Similar(13)
We note, again, that with our alignment of mitochondrial proteins, we exclude sites between a gap and a site that is constant across the dataset, and if we applied this conservative alignment criterion, we would have to exclude all FGB-int7 sites.
Taking the partial derivatives with respect to α, we can obtain the following solution: (8) α = (V W T V W + λ Θ ) - 1 (V W T V K + λ μ ) It is easy to see that, if λ = 0, only the kernel target alignment criterion is used to optimize α, and MNet is similar to SW.
Reads that passed the alignment quality criteria were realigned to local reference genome fragments using Cross_match software; the refined alignments were parsed for sequence variation between the subject and the reference.
However, alignment coverage criteria failed for many hits despite good E-values.
For the second (Type2.2) and third (Type2.3) sets alignment identity criteria was relaxed to 50 and 35%% respectively [ 41].
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