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The sections were air-dried completely and imaged using a Zeiss Axioplan II upright microscope.
Thin smears was prepared on the slides and air-dried completely at room temperature.
They were mounted with Permount and air-dried completely before imaging under Zeiss Axioplan II upright microscope.
Fixative agent was removed and wells air-dried completely prior to staining.
The bead pellet was air-dried completely and the size-selected sequencing library eluted by resuspending the pellet in 53 μL of TE buffer (10 mM Tris (pH 8.0), 1 mM EDTA (ph 8.0)).
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The slide was air-dried almost completely and then covered with 400 µl of fixative (3:1 [v/v] methanol/glacial acetic acid).
Slides were rinsed twice 10 min in 1 × PBS at room temperature, and completely air-dried.
Extracts were then filtered through Whatman No. 1 filter paper, concentrated in vacuo at 40 °C and completely air-dried at room temperature in glass vials.
The sections were then immersed in 0.0005% FJB (Millipore, Billerica, MA, USA) in a dark room for 30 min, after which they were completely air-dried before being immersed in xylene and enclosed in malinol (Muto Pure Chemicals, Tokyo Japan).
After vortexing, the mixture was centrifuged at 15,000 g for 3 min. The pellet was completely air-dried, resuspended in 100 μL TE, and centrifuged at 15,000 g for 3 min. One microliter of the supernatant was used as template in a 20-μL colony PCR reaction.
All air-dried seeds were collected and kept at 4 °C.
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