After stimulating the rats' central thalamus for 30 minutes, Herrera and colleagues found that two genes--one linked to neural activity and the other linked to cellular mechanisms of learning--had become more active in the rats' brains, including in the cerebral cortex and hippocampus.
After stimulating transfected mast cells with both 10−7M Phorbol 12-Myristate 13-Acetate (PMA) and 10−6M ionomycin, we analyzed the release of β-hexosaminidase, a lysosomal enzyme stored inside mast cell secretory lysosomes.
After stimulating the HeLa cells for 10 min with CXCL12/SDF-1, immunopreCXCR4ating CXCR4 (anti-GFP andibody) and re-blotting for β-Arrestin 2 (anti-FLAG antibody), we detected β-Arrestin 2 (βarr2) in immunoprecipitates from HeLa cells transduced with the WT receptor, but not from HeLa cells transduced with the WHIM mutant receptor (Figure 4A).
After stimulating the cells for 14 and 21 days, ALP expression and cell mineralization were strongest (Figs. 2, 3, 4).
After stimulating with LPS or TNF-α for indicated time periods and concentrations, 20 μl of MTT Sigma Chemical Coo).
After stimulating PBMCs for 4 hours with PMA and ionomycin, we identified CD4+ T cells producing IL-17, IFN-γ, or both, by using flow cytometry [ 27].
After stimulating IgE-primed mast cells with 25 ng/ml antigen for 7 min, Lyn or Fyn were immunoprecipitated from whole cell lysates by using specific antibodies.
After stimulating B cells in vitro, we detected a higher IgG4/IgG total ratios in CXCL13 treated group compared with that in dexamethasone group or control.
After stimulating mBMMCs via FcϵRI cross-linking, we found that the secretion of histamine, which is released by regulated exocytosis, was reduced by ~50% in cells from heterozygous mutant mice compared with those from WT mice.
However, these lysates of the shattered S. aureus only marginally activated NF-κB in SM10 (Fig. 3c).> -wrap-foot> Varees are the ratio of increased mRNA concentration (fold over control) after 3 h stimulation with 30 μg/ml of RiboLyser lysates and the corresponding value obtained after stimulating with 30 μg/ml of the respective pathogen particles.
A suitable cell substrate (CS) for isolating such PHIV is peripheral blood mononuclear cells (PBMC) after stimulating with phytohemagglutinin (PHA) and interleukin-2 (IL-2).
