After sequence of permutation process, the entire speech samples are appended.
After sequence analysis on 96 randomly picked positive colonies, 24 (25%) of the colonies were found to contain microsatellite sequences, and 20 primer pairs were designed.
After sequence identification, GPR81 was inserted into the plasmid pcDNA3.1/his-mycA and then transfected into Chinese hamster ovary cell (CHO-K1).
After sequence verification and LguI/Eco81I double digestion, genes were ligated into LguI-linearized pQE-30-LE to give plasmid pQE-30-LE::bgl1 (Eurofins, Ebersberg, Germany).
After sequence verification, the HvNAS1-tNOS fragment was digested with SpeI, whereas the OsActin1 promoter in the SK vector was digested with XbaI.
After sequence design, the resulting structure is minimimized.
After sequence confirmation of all four PCR products, one clone was selected for aggregation.
After sequence confirmation, altered msn DNAs were sub-cloned into the pH Stinger vector.
After sequence verification, the construct was used to transform the ΔMosec22 knockout mutants.
After sequence preparation, gene families were constructed and orthologous relationships were identified by TreeFam [62] method (see Methods S1).
After sequence confirmation, fragments were recloned to murine stem cell virus (MSCV -based MSCV -basedor for retroviral expression.
