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After mix, the sample were centrifuged for 20 min at 20,000 x g, and the supernatant was mixed well with ice-cold acetone (1 4, v/ v) with 30 mM DTT.
And so, as he plays mix after mix after mix, month after month after month, Shane stares out at the thousands of Europeans who will soon be flying home, and wishes he could one day go with them.
The "music for the evening after" mix showcases what I consider to be the more oblique, psychedelic side of house and techno, where dance-floor insistence gives way to something more ruminative, and forward motion gives way to eddies and crosscurrents.
Here at THUMP UK HQ, we spend most of our working days listening to mix after mix.
Watching Hood at work is an exercise in transcendence, and after all, isn't that why we go to clubs and festivals, why we listen to mix after mix, record after record to find something above and beyond us?
Bwana's approach to music is not to inundate the scene with track after track, mix after mix and get lost in the hype, instead, he looks to artists like Midland and Montreal's Jacques Greene and observes how they've administered their music velocity.
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Pairing with the winner of an Arc, which is run over a mile and a half, could potentially produce a much sought-after mix of speed and stamina.
After mixing with ASW, the mixed aqueous solution was continuously stirred.
Prospective before-after, mixed-methods (quantitative and qualitative) study.
After mixing, samples were incubated for 1 h at 25 °C.
After mixing for 30 min, NEWMS were prepared.
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