After labelling, the membranes were washed in TBST and then incubated with a phosphatase-labeled second antibody (Jackson Immuno Research Laboratory, appropriate dilution).
After labelling, unincorporated dye was removed using RNeasy (Qiagen, Valencia, CA) mini-spin columns.
After labelling all the images, each head has been automatically registered in order to assure scale and rotation invariance.
After labelling the biotinylated hybrid with a streptavidin enzyme conjugate, the electrochemical detection of the enzymatic product was performed onto the surface of disposable carbon nanotube-modified electrode.
After labelling in the presence of ascorbic acid under the standard procedure, an incorporation of >99 % and a RCP of > 85%% were found, Fig. 3a.
After labelling, unincorporated dye was removed by purification with a Qiagen PCR purification column.
After labelling the cells were embedded in Mowiol containing Dabco antifade reagent (Sigma).
After labelling, red blood cells were lysed and fixed.
After labelling, TRAP-positive cells were subjected to DIC microscopy.
After labelling, the cells were washed and analysed by flow cytometry (FacsCalibur, BD Pharmingen).
After labelling, lymphocytes were incubated at 37°C in a 5% CO2 incubator for 96 h.
