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After insemination for 2 h, eggs were washed out of surface bound sperm and fixed for immunofluorescence study.
After insemination, the female's behavior changes from mate-searching to host-fruit location for oviposition [ 64].
Furthermore, we tested whether L. stagnalis becomes reluctant to inseminate a mate after being inseminated because it is known that after insemination, male investment substantially reduces.
In our study, virgins and inseminated queens were age-matched, and all queens were collected 5 days after insemination and were not laying eggs.
Pregnancy status was determined 33 ± 3 d after insemination and was reconfirmed 63 ± 3 d after insemination.
At d 7 after insemination, embryos were transferred fresh or following vitrification.
The duration of hatchability was 4 6 weeks after insemination for four of the turkey lines.
In summary, jennies are susceptible to endometritis after insemination with frozen-thawed semen.
All but one piglet born after insemination with sexed semen were of the predicted sex.
Embryos were collected at 48 h and day 5 after insemination.
Pregnancy diagnosis was determined by transrectal ultrasonography 40 days after insemination.
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CEO of Professional Science Editing for Scientists @ prosciediting.com