(1) After flushing bone marrow out of the marrow cavity, we cultured the cells with fat mass without filtering and washing them.
After flushing with ambient air, the tube air equilibrates with soil air through the membrane underside allowing direct soil efflux calculations.
After flushing the bottles with CH4-free N2 and autoclaving, the headspace gas concentration was measured in order to get initial point analyses.
After flushing the serum bottles, they were sealed with butyl rubber stoppers and aluminum crimps (Bellco Glass, Inc., Vineland, NJ, USA).
After flushing the lumen, the tissue was homogenized in phosphate-buffered saline (PBS) buffer with ethylene diamine tetra-acetic acid (EDTA).
After flushing them out, the castle mistress Rose offers her service to the Overlord.
After flushing with nitrogen, this mixture was heated for 30 min at 70°C in closed glass tubes.
After flushing and cleaning, the implantation level of the screw was verified using a small trial button.
After flushing with isotonic saline kept at body temperature, the intestine was once again lifted and held by the supporting device.
After flushing rapidly with saline, the mucosa was scraped off with a spatula and immediately frozen in liquid nitrogen for protein analyses.
After flushing with CO2, screw capped and incubated for 1, 2 and 3 h in a water bath with shaker at 39°C (Stalproducter, Uppasala, Sweden).
