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After alignment, ambiguous regions (i.e. containing gaps and/or poorly aligned) were removed with Gblocks (v0.91b).
After alignment of both sequences, specific primers pairs were designed from variable or unchanging nucleotide segments.
After alignment of nucleotide sequences of both species, specific primer sets were designed from unchanging and variable gene segments.
After alignment, we found out that the mei samples contained 18 nucleotide gaps, whereas none in the plums.
After alignment, automatic spot matching was performed.
After alignment, preliminary three-dimensional maps were calculated using a weighted back projection algorithm.
After alignment and tree analysis of complete DBL6ε domain sequences, no consensus sequence is evident.
After alignment, the molecules were placed in a 3-D cubic lattice with 2-Å spacing.
After alignment, masks were collated into a single stack and averaged by calculating a mean intensity projection.
After alignment the positions where gaps occurred were stripped and minor manual adjustments were made using MacClade [33].
After alignment, each image was segmented into discrete components by manually tracing every structure of interest (Fig. 10C).
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